UNIT 18.1 Binding of Biotinylated Peptides to MHC Class II Proteins on Cell Surfaces
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Rothbard, J. B. and Busch, R. 2001. Binding of Biotinylated Peptides to MHC Class II Proteins on Cell Surfaces. Current Protocols in Immunology. 25:18.1:18.1.1–18.1.15.
- Published Online: 1 MAY 2001
- Published Print: MAR 1998
This unit describes a simple, reproducible, and semiquantitative assay for measuring ligand binding to cell surface receptors. This approach is useful as a quick screen for peptide binding to MHC class II proteins that avoids the need to purify the MHC class II molecules and that uses small numbers of cells. The describes procedures for incubating cells expressing the MHC molecule of interest with a biotinylated peptide, washing off excess peptide, and detecting the bound peptides by staining with fluorescently labeled avidin. Bound fluorescence is then quantitated by flow cytometry. An provides a more sensitive method of measuring the peptide-receptor complexes, and is useful when receptor density or binding site availability become limiting factors in the . The uses an avidin/anti-avidin/avidin sandwich, which has been found to increase sensitivity substantially without sacrificing specificity. Support protocols are provided for biotinylation of synthetic peptides in solution and for preparation of peptide stock solutions for use in the binding assays.