UNIT 18.6 Affinity-Based Biosensors for Biomolecular Interaction Analysis

  1. Kerstin Gunnarsson

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1806s33

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Gunnarsson, K. 2001. Affinity-Based Biosensors for Biomolecular Interaction Analysis. Current Protocols in Immunology. 33:18.6:18.6.1–18.6.10.

Author Information

  1. BIACORE AB, Uppsala, Sweden

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1999


This overview presents an introduction to affinity-based biosensors, most notably the BIACORE. These biosensors detect molecular interactions by immobilizing one of the interactants (the ligand or target molecule) on the surface of a sensor chip. A solution containing the other interactant is then applied to the chip. Binding of analyte to the surface results in a response proportional to the mass bound. A discussion of sensor chips and practical applications is also provided.