UNIT 18.6 Affinity-Based Biosensors for Biomolecular Interaction Analysis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Gunnarsson, K. 2001. Affinity-Based Biosensors for Biomolecular Interaction Analysis. Current Protocols in Immunology. 33:18.6.1–18.6.10.
- Published Online: 1 MAY 2001
- Published Print: OCT 1999
This overview presents an introduction to affinity-based biosensors, most notably the BIACORE. These biosensors detect molecular interactions by immobilizing one of the interactants (the ligand or target molecule) on the surface of a sensor chip. A solution containing the other interactant is then applied to the chip. Binding of analyte to the surface results in a response proportional to the mass bound. A discussion of sensor chips and practical applications is also provided.