Unit

UNIT 18.7 Biotin Labeling and Quantitation of Cell-Surface Proteins

  1. Diane N. Turvy,
  2. Janice S. Blum

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1807s36

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Turvy, D. N. and Blum, J. S. 2001. Biotin Labeling and Quantitation of Cell-Surface Proteins. Current Protocols in Immunology. 36:18.7:18.7.1–18.7.12.

Author Information

  1. Indiana University School of Medicine, Indianapolis, Indiana

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000

Abstract

Cell surface receptors, such as transferrin receptors and MHC molecules, are internalized into the endocytic pathway and recycled to the plasma membrane. Previous assays used to measure endocytosis and recycling were cumbersome and often required radioactive reagents. This unit describes protocols that employ the combination of a cleavable biotin reagent to label surface molecules and a capture ELISA to detect these molecules allowing for rapid and safe quantitation of cell surface protein expression, endocytosis, and recycling.