UNIT 18.7 Biotin Labeling and Quantitation of Cell-Surface Proteins
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Turvy, D. N. and Blum, J. S. 2001. Biotin Labeling and Quantitation of Cell-Surface Proteins. Current Protocols in Immunology. 36:18.7:18.7.1–18.7.12.
- Published Online: 1 MAY 2001
- Published Print: APR 2000
Cell surface receptors, such as transferrin receptors and MHC molecules, are internalized into the endocytic pathway and recycled to the plasma membrane. Previous assays used to measure endocytosis and recycling were cumbersome and often required radioactive reagents. This unit describes protocols that employ the combination of a cleavable biotin reagent to label surface molecules and a capture ELISA to detect these molecules allowing for rapid and safe quantitation of cell surface protein expression, endocytosis, and recycling.