UNIT 18.8 Measuring Protein-Protein Interactions by Equilibrium Sedimentation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Schuck, P. and Braswell, E. H. 2001. Measuring Protein-Protein Interactions by Equilibrium Sedimentation. Current Protocols in Immunology. 18:18.8.
- Published Online: 1 MAY 2001
- Published Print: DEC 2000
This is not the most recent version of the article. View current version (1 NOV 2007)
This commentary unit provides a basic introduction to the principles and practice of sedimentaiton equilibrium analytical ultracentrifugation for the study of reversible protein interactions. Equilibrium sedimentation is one of the most effective methods for the detection and characterization of protein interactions and can be applied to the measurement of self association, heterologous association, binding stoichiometry, and the determination of association constants. The unit includes an extensive discussion of the instrumentation required to carry out equilibrium analytical ultracentrifugation and to perform data analysis. A strategic planning section gives the reader several experimental scenarios and conditions for using equilibrium sedimentation.