UNIT 18.8 Measuring Protein-Protein Interactions by Equilibrium Sedimentation
Published Online: 1 NOV 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Balbo, A., Brown, P. H., Braswell, E. H. and Schuck, P. 2007. Measuring Protein-Protein Interactions by Equilibrium Sedimentation. Current Protocols in Immunology. 79:18.8:18.8.1–18.8.28.
- Published Online: 1 NOV 2007
- Published Print: NOV 2007
This unit describes basic principles and practice of sedimentation equilibrium analytical ultracentrifugation for the study of reversible protein interactions, such as the characterization of self-association, heterogeneous association, and binding stoichiometry, as well as the determination of association constants. Advanced tools such as mass conservation analysis, multiwavelength analysis, and global analysis are introduced and discussed in the context of the experimental design. A detailed protocol guiding the investigator through the experimental steps and the data analysis is available as an internet resource. Curr. Protoc. Immunol. 79:18.8.1-8.18.28. © 2007 by John Wiley & Sons, Inc.
- sedimentation equilibrium;
- sedimentation velocity;
- chemical equilibria;
- reversible interactions;
- multi-protein complex;
- analytical ultracentrifugation