UNIT 18.9 Binding of Small Peptides to Immobilized Antibodies: Kinetic Analysis by Surface Plasmon Resonance
Published Online: 1 NOV 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Andreu, D. and Gomes, P. 2002. Binding of Small Peptides to Immobilized Antibodies: Kinetic Analysis by Surface Plasmon Resonance. Current Protocols in Immunology. 50:18.9:18.9.1–18.9.22.
- Published Online: 1 NOV 2002
- Published Print: AUG 2002
This unit describes a method for screening small viral peptides as specific antigens using a surface plasmon resonance (SPR) biosensor. The in this unit is suited for direct single-step SPR analysis of small ligand-large receptor interactions, where small peptides are used as analytes (injected in the continuous buffer flow) and monoclonal antibodies (MAbs) are immobilized on the SPR sensor chip surface. An is included for situations where kinetic analysis is not possible and uses a surface competition assay to indirectly measure the kinetics of small analyte binding.