Unit

UNIT 18.9 Binding of Small Peptides to Immobilized Antibodies: Kinetic Analysis by Surface Plasmon Resonance

  1. David Andreu1,
  2. Paula Gomes2

Published Online: 1 NOV 2002

DOI: 10.1002/0471142735.im1809s50

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Andreu, D. and Gomes, P. 2002. Binding of Small Peptides to Immobilized Antibodies: Kinetic Analysis by Surface Plasmon Resonance. Current Protocols in Immunology. 50:18.9:18.9.1–18.9.22.

Author Information

  1. 1

    Universitat Pompeu Fabra, Barcelona, Spain

  2. 2

    Centro de Investigação em Química da Universidade do Porto, Porto, Portugal

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: AUG 2002

Abstract

This unit describes a method for screening small viral peptides as specific antigens using a surface plasmon resonance (SPR) biosensor. The basic protocol in this unit is suited for direct single-step SPR analysis of small ligand-large receptor interactions, where small peptides are used as analytes (injected in the continuous buffer flow) and monoclonal antibodies (MAbs) are immobilized on the SPR sensor chip surface. An alternate protocol is included for situations where kinetic analysis is not possible and uses a surface competition assay to indirectly measure the kinetics of small analyte binding.