Unit

UNIT 18.10 Fluorescence Resonance Energy Transfer: Techniques for Measuring Molecular Conformation and Molecular Proximity

  1. Michael Edidin

Published Online: 1 FEB 2003

DOI: 10.1002/0471142735.im1810s52

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Edidin, M. 2003. Fluorescence Resonance Energy Transfer: Techniques for Measuring Molecular Conformation and Molecular Proximity. Current Protocols in Immunology. 57:18.10:18.10.1–18.10.18.

Author Information

  1. Johns Hopkins University, Baltimore, Maryland

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: DEC 2002

Abstract

This overview unit focuses on the basics of fluorescence and of the FRET phenomenon, and on methods for detecting FRET and data interpretation. FRET is very versatile and there are more application of these basics than can be covered in a single overview. However, some examples are given of applications of various FRET techniques.