UNIT 18.14 Measurement of Peptide Dissociation from MHC Class II Molecules

  1. Francisco A. Chaves,
  2. Andrea J. Sant

Published Online: 1 MAY 2007

DOI: 10.1002/0471142735.im1814s77

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Chaves, F. A. and Sant, A. J. 2007. Measurement of Peptide Dissociation from MHC Class II Molecules. Current Protocols in Immunology. 77:18.14:18.14.1–18.14.11.

Author Information

  1. University of Rochester, Rochester, New York

Publication History

  1. Published Online: 1 MAY 2007
  2. Published Print: MAY 2007


This unit describes a method of measuring the kinetic stability of complexes formed between purified MHC class II molecules and antigenic N-terminal fluorescein-labeled peptides. An HPLC-SEC allows for the separation of fluoresceinated MHC class II:peptide complexes from excess free dissociating peptides, while the fluorescence detector allows for the quantification of the remaining complexes throughout the dissociation time-course.


  • Peptide;
  • MHC class II molecules;
  • epitope;
  • immunodominance;
  • vaccine design;
  • dissociation assay