UNIT 18.14 Measurement of Peptide Dissociation from MHC Class II Molecules
Published Online: 1 MAY 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Chaves, F. A. and Sant, A. J. 2007. Measurement of Peptide Dissociation from MHC Class II Molecules. Current Protocols in Immunology. 77:18.14:18.14.1–18.14.11.
- Published Online: 1 MAY 2007
- Published Print: MAY 2007
This unit describes a method of measuring the kinetic stability of complexes formed between purified MHC class II molecules and antigenic N-terminal fluorescein-labeled peptides. An HPLC-SEC allows for the separation of fluoresceinated MHC class II:peptide complexes from excess free dissociating peptides, while the fluorescence detector allows for the quantification of the remaining complexes throughout the dissociation time-course.
- MHC class II molecules;
- vaccine design;
- dissociation assay