UNIT 18.16 Generation of Peptide MHC Class I Monomers and Multimers Through Ligand Exchange
Published Online: 1 NOV 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Toebes, M., Rodenko, B., Ovaa, H. and Schumacher, T. N. 2009. Generation of Peptide MHC Class I Monomers and Multimers Through Ligand Exchange. Current Protocols in Immunology. 87:18.16:18.16.1–18.16.20.
- Published Online: 1 NOV 2009
- Published Print: NOV 2009
The recognition of defined antigen-MHC complexes by antigen-specific T cells forms the molecular basis of T cell immunity. It has been shown that fluorescently labeled recombinant MHC tetramers can be utilized to detect antigen-specific T cells by flow cytometry. Since this first description, MHC tetramers and other types of MHC multimers have become a core tool to monitor the development of disease- and therapy-induced antigen-specific T cell responses both in humans and in animal model systems. This unit describes a set of protocols that transform classical MHC multimer technology into a high-throughput platform, allowing one to produce large collections of MHC class I molecules charged with different peptides. This technology is based on the development of conditional MHC ligands that can be triggered to self-destruct while in the MHC-bound state. Curr. Protoc. Immunol. 87:18.16.1-18.16.20. © 2009 by John Wiley & Sons, Inc.
- HLA class I;
- MHC class I;