Unit

UNIT 18.18 Analysis of the Targeting Properties of Fc Chimeric Proteins and Antibodies in Mice In Vivo

  1. Luisa Martinez-Pomares

Published Online: 1 APR 2012

DOI: 10.1002/0471142735.im1818s97

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Martinez-Pomares, L. 2012. Analysis of the Targeting Properties of Fc Chimeric Proteins and Antibodies in Mice In Vivo. Current Protocols in Immunology. 97:18.18:18.18.1–18.18.12.

Author Information

  1. Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, United Kingdom

Publication History

  1. Published Online: 1 APR 2012
  2. Published Print: APR 2012

Abstract

This unit includes protocols for the analysis of the targeting properties of Fc chimeric proteins and antibodies in mice in vivo. We present procedures to prepare the reagents to be tested under endotoxin-free conditions, suitable injection sites, methodology for tissue collection, and processing, and labeling procedures to detect the injected material in situ. Curr. Protoc. Immunol. 97:18.18.1-18.18.12. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • macrophages;
  • antibodies;
  • chimeric Fc proteins