UNIT 19.2 Animal Models for the Analysis of Immune Responses to Leishmaniasis

  1. David L. Sacks1,
  2. Peter C. Melby2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1902s28

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Sacks, D. L. and Melby, P. C. 2001. Animal Models for the Analysis of Immune Responses to Leishmaniasis. Current Protocols in Immunology. 28:19.2:19.2.1–19.2.20.

Author Information

  1. 1

    National Institute of Allergy & Infectious, Diseases, Bethesda, Maryland

  2. 2

    University of Texas Health Sciences Center, San Antonio, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1998

This is not the most recent version of the article. View current version (2 FEB 2015)


This unit focuses on the murine model of cutaneous leishmaniasis and models of visceral leishmaniasis in mice and hamsters. Each basic protocol describes the methods used to inoculate parasites and to evaluate infections with regard to lesion progression and visceralization, and quantification of parasite load. Five support protocols are provided; two for the growth and isolation of metacyclic promastigotes from in vitro culture, one for isolation of tissue amastigotes from infected animals, one for cryopreservation of parasites, and one for the preparation of blood agar plates for quantitation of parasite numbers in infected tissue.