UNIT 19.2 Animal Models for the Analysis of Immune Responses to Leishmaniasis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Sacks, D. L. and Melby, P. C. 2001. Animal Models for the Analysis of Immune Responses to Leishmaniasis. Current Protocols in Immunology. 28:19.2:19.2.1–19.2.20.
- Published Online: 1 MAY 2001
- Published Print: DEC 1998
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This unit focuses on the murine model of cutaneous leishmaniasis and models of visceral leishmaniasis in mice and hamsters. Each basic protocol describes the methods used to inoculate parasites and to evaluate infections with regard to lesion progression and visceralization, and quantification of parasite load. Five support protocols are provided; two for the growth and isolation of metacyclic promastigotes from in vitro culture, one for isolation of tissue amastigotes from infected animals, one for cryopreservation of parasites, and one for the preparation of blood agar plates for quantitation of parasite numbers in infected tissue.