UNIT 19.4 Experimental Asexual Blood Stage Malaria Immunity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Amante, F. H. and Good, M. F. 2001. Experimental Asexual Blood Stage Malaria Immunity. Current Protocols in Immunology. 29:19.4:19.4.1–19.4.18.
- Published Online: 1 MAY 2001
- Published Print: FEB 1999
This is not the most recent version of the article. View current version (1 APR 2011)
This unit describes three methods for investigating the immune response to murine malaria parasites. Immunization protocols using recombinant fragments of the merozoite surface protein-1 of Plasmodium yoelii, whole blood stage malaria parasites, and live infection with parasitized red blood cells from a Plasmodium-infected donor are provided. Methods for chemotherapeutic drug care of Plasmodium-infected mice and for inducing malaria by adoptive transfer of antigen-specific T cells are included. Finally, support protocols describe methods for growing, maintaining, and cryopreserving murine asexual blood stage malaria parasites and for preparing blood stage antigen for use in ELISAs.