UNIT 19.5 Animal Models of Mycobacteria Infection

  1. Diane J. Ordway,
  2. Ian M. Orme

Published Online: 1 AUG 2011

DOI: 10.1002/0471142735.im1905s94

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Ordway, D. J. and Orme, I. M. 2011. Animal Models of Mycobacteria Infection. Current Protocols in Immunology. 94:19.5:19.5.1–19.5.50.

Author Information

  1. Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado

Publication History

  1. Published Online: 1 AUG 2011
  2. Published Print: AUG 2011


This unit describes the infection of mice and guinea pigs with mycobacteria via various routes, as well as necropsy methods for the determination of mycobacterial loads within target organs. Additionally, methods for cultivating mycobacteria and preparing stocks are described. The protocols outlined are primarily used for M. tuberculosis, but can also be used for the study of other non-tuberculosis mycobacterial species. Curr. Protoc. Immunol. 94:19.5.1-19.5.50. © 2011 by John Wiley & Sons, Inc.


  • mycobacteria;
  • tuberculosis;
  • mouse;
  • guinea pig;
  • inhalation exposure system;
  • necropsy;
  • animal models