Unit

UNIT 19.7 A Mouse Model for Cytomegalovirus Infection

  1. Wolfram Brune,
  2. Hartmut Hengel,
  3. Ulrich H. Koszinowski

Published Online: 1 AUG 2001

DOI: 10.1002/0471142735.im1907s43

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Brune, W., Hengel, H. and Koszinowski, U. H. 2001. A Mouse Model for Cytomegalovirus Infection. Current Protocols in Immunology. 43:19.7:19.7.1–19.7.13.

Author Information

  1. University of Munich, Max von Pettenkofer Institute, Munich, Germany

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: JUN 2001

Abstract

This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (mCMV). Methods are included for propagating mCMV in cell cultures and for preparing a more virulent form of mCMV from salivary glands of infected mice. A plaque-forming cell (PFC) assay is provided for measuring mCMV titers of infected tissues or virus stocks. In addition, a method is described for preparing the murine embryonic fibroblasts used for propagating mCMV and for the PFC assay.