UNIT 19.7 A Mouse Model for Cytomegalovirus Infection
Published Online: 1 AUG 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Brune, W., Hengel, H. and Koszinowski, U. H. 2001. A Mouse Model for Cytomegalovirus Infection. Current Protocols in Immunology. 43:19.7:19.7.1–19.7.13.
- Published Online: 1 AUG 2001
- Published Print: JUN 2001
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (mCMV). Methods are included for propagating mCMV in cell cultures and for preparing a more virulent form of mCMV from salivary glands of infected mice. A plaque-forming cell (PFC) assay is provided for measuring mCMV titers of infected tissues or virus stocks. In addition, a method is described for preparing the murine embryonic fibroblasts used for propagating mCMV and for the PFC assay.