UNIT 19.10 Animal Models Using Lymphocytic Choriomeningitis Virus
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
von Herrath, M. and Whitton, J. L. 2001. Animal Models Using Lymphocytic Choriomeningitis Virus. Current Protocols in Immunology. 36:19.10:19.10.1–19.10.19.
- Published Online: 1 MAY 2001
- Published Print: APR 2000
This unit includes protocols for inducing systemic infection and persistent infection of mice with lymphocytic choriomeningitis virus (LCMV). Methods used to measure T cell responses to LCMV are then described. A protocol to assess anti-LCMV immunity in vivo is also included. Support protocols for preparing LCMV stocks and measuring LCMV titers using a plaque assay are also included. Finally, a support protocol for detecting anti-LCMV antibodies by ELISA is presented.