UNIT 19.12 Animal Model of Nippostrongylus brasiliensis and Heligmosomoides polygyrus
Published Online: 1 AUG 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Camberis, M., Le Gros, G. and Urban, J. 2003. Animal Model of Nippostrongylus brasiliensis and Heligmosomoides polygyrus. Current Protocols in Immunology. 55:19.12.1–19.12.27.
- Published Online: 1 AUG 2003
- Published Print: JUN 2003
Animal models of Nippostrongylus brasiliensis and Heligmosomoides polygyrus infection are powerful tools for the investigation of the basic biology of immune responses and protective immunity. In particular, they model the induction and maintenance of Th2 type immune responses and exhibit all the requisite hallmarks of CD4 T celldependent IgE production, eosinophilia, mastocytosis, and mucus production. This chapter describes simple, cost-effective techniques for using and maintaining these easy-to-work-with parasites in the context of a modern laboratory.
Keywords: immunoparasitology; nematodes; Nippostrongylus brasiliensis; Heligmosomoides polygyrus; Th2 immune responses; cytokines; mucosal immunity; allergic responses; IL4; IL5; IL9; IL13; IgE; eosinophils; mastocytosis; protective immunity; worm migration; parasitic diseases; inflammatory responses; nematode biology