UNIT 19.13 Cecal Ligation and Puncture

  1. Alex G. Cuenca,
  2. Matthew J. Delano,
  3. Kindra M. Kelly-Scumpia,
  4. Lyle L. Moldawer,
  5. Philip A. Efron

Published Online: 1 NOV 2010

DOI: 10.1002/0471142735.im1913s91

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Cuenca, A. G., Delano, M. J., Kelly-Scumpia, K. M., Moldawer, L. L. and Efron, P. A. 2010. Cecal Ligation and Puncture. Current Protocols in Immunology. 91:19.13:19.13.1–19.13.11.

Author Information

  1. University of Florida College of Medicine, Gainesville, Florida

Publication History

  1. Published Online: 1 NOV 2010
  2. Published Print: NOV 2010


The cecum contains a high concentration of microbes, which are a combination of Gram-negative and Gram-positive flora. These bacteria range from anaerobic to facultative aerobic to aerobic organisms. In the procedure described in this unit, the ligation of the cecum produces a source of ischemic tissue as well as polymicrobial infection. This combination of ischemic/necrotic tissue and microbial infection distinguishes this multifactorial model from a number of other bacterial sepsis models, including but not limited to: bacteremia secondary to intravenous or intraperitoneal administration; fecal administration or intraperitoneal administration of fecal or bacterial plugs; colonic stents; and bacterial abscess formation. Curr. Protoc. Immunol. 91:19.13.1-19.13.11. © 2010 by John Wiley & Sons, Inc.


  • sepsis;
  • cecal ligation and puncture;
  • systemic inflammatory response syndrome;
  • shock;
  • murine model