UNIT 19.13 Cecal Ligation and Puncture
Published Online: 1 NOV 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Cuenca, A. G., Delano, M. J., Kelly-Scumpia, K. M., Moldawer, L. L. and Efron, P. A. 2010. Cecal Ligation and Puncture. Current Protocols in Immunology. 91:19.13.1–19.13.11.
- Published Online: 1 NOV 2010
- Published Print: NOV 2010
The cecum contains a high concentration of microbes, which are a combination of Gram-negative and Gram-positive flora. These bacteria range from anaerobic to facultative aerobic to aerobic organisms. In the procedure described in this unit, the ligation of the cecum produces a source of ischemic tissue as well as polymicrobial infection. This combination of ischemic/necrotic tissue and microbial infection distinguishes this multifactorial model from a number of other bacterial sepsis models, including but not limited to: bacteremia secondary to intravenous or intraperitoneal administration; fecal administration or intraperitoneal administration of fecal or bacterial plugs; colonic stents; and bacterial abscess formation. Curr. Protoc. Immunol. 91:19.13.1-19.13.11. © 2010 by John Wiley & Sons, Inc.
- cecal ligation and puncture;
- systemic inflammatory response syndrome;
- murine model