UNIT 20.6 Models for Lymphoma

  1. Arya Biragyn,
  2. Larry W. Kwak

Published Online: 1 FEB 2002

DOI: 10.1002/0471142735.im2006s46

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Biragyn, A. and Kwak, L. W. 2002. Models for Lymphoma. Current Protocols in Immunology. 46:20.6:20.6.1–20.6.30.

Author Information

  1. National Cancer Institute, Frederick, Maryland

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: DEC 2001


This unit describes experimental procedures for development of therapeutic vaccines, particularly “second-generation” recombinant vaccines. Specifically, a general procedure for handling and culturing lymphoma cell lines in vitro and their subsequent challenge into syngeneic mice is described. Several protocols describe the production of various idiotype (Id)-based or cellular lymphoma vaccine formulations. In particular, the novel approach of rendering nonimmunogenic lymphoma-derived scFv or Id immunogenic by fusing it with a chemokine moiety is described. As an alternative, a protocol for expression and purification of these chemokine-fusion proteins from E. coli is included. A general procedure is included for cloning of cytokine genes, for example, murine GM-CSF, in A20 lymphoma cells. Support protocols are presented for chemically conjugating intact Ig protein with KLH to produce a prototype protein vaccine and using the Helios Gene Gun System to immunize mice with recombinant DNA tumor vaccines.