Unit

UNIT 20.10 Molecular Cloning and Characterization of MHC Class I– and II–Restricted Tumor Antigens Recognized by T Cells

  1. Rong-Fu Wang

Published Online: 1 FEB 2009

DOI: 10.1002/0471142735.im2010s84

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Wang, R.-F. 2009. Molecular Cloning and Characterization of MHC Class I– and II–Restricted Tumor Antigens Recognized by T Cells. Current Protocols in Immunology. 84:20.10:20.10.1–20.10.29.

Author Information

  1. The Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, Texas

Publication History

  1. Published Online: 1 FEB 2009
  2. Published Print: FEB 2009

Abstract

T cells play a central role in cancer immunosurveillance, autoimmune, and infectious diseases. Identification of MHC class I– and II–restricted T cell peptides is a critical step for the development of effective vaccines against cancer and infectious diseases. This unit describes a cDNA expression system and a genetic targeting expression system for the cloning of genes encoding for MHC class I– and II–restricted antigens recognized by antigen-specific CD8+ and CD4+ T cells. Curr. Protoc. Immunol. 84:20.10.1-20.10.29. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • T helper peptides;
  • tumor antigens;
  • cancer vaccines;
  • immunotherapy;
  • CD4+ T cells;
  • cDNA library;
  • targeting expression;
  • MHC class II