UNIT 21.1 Proper Alignment and Adjustment of the Light Microscope
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Salmon, E. D. and Canman, J. C. 2002. Proper Alignment and Adjustment of the Light Microscope. Current Protocols in Immunology. 48:21.1.1–21.1.26.
- Published Online: 1 MAY 2002
- Published Print: APR 2002
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications (e.g., brightfield, phase-contrast, differential interference contrast, and fluorescence epi-illumination), and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The principles of microscopy and step-by-step alignment and adjustment procedures are described in this unit.