UNIT 21.2 Fluorescence Microscopy

  1. Brian Herman

Published Online: 1 MAY 2002

DOI: 10.1002/0471142735.im2102s48

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Herman, B. 2002. Fluorescence Microscopy. Current Protocols in Immunology. 48:21.2:21.2.1–21.2.10.

Author Information

  1. University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: APR 2002


Absorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular structures. This unit discusses the optics and other components of the fluorescence microscope, their functions and adjustments, and the digital darkroom.