UNIT 21.2 Fluorescence Microscopy
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Herman, B. 2002. Fluorescence Microscopy. Current Protocols in Immunology. 48:21.2:21.2.1–21.2.10.
- Published Online: 1 MAY 2002
- Published Print: APR 2002
Absorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular structures. This unit discusses the optics and other components of the fluorescence microscope, their functions and adjustments, and the digital darkroom.