UNIT 21.3 Immunofluorescence Staining

  1. Julie G. Donaldson

Published Online: 1 MAY 2002

DOI: 10.1002/0471142735.im2103s48

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Donaldson, J. G. 2002. Immunofluorescence Staining. Current Protocols in Immunology. 48:21.3:21.3.1–21.3.6.

Author Information

  1. National Heart, Lung, and Blood Institute/NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: APR 2002


The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications (e.g., brightfield, phase-contrast, differential interference contrast, and fluorescence epi-illumination), and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The principles of microscopy and step-by-step alignment and adjustment procedures are described in this unit.