UNIT 21.3 Immunofluorescence Staining
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Donaldson, J. G. 2002. Immunofluorescence Staining. Current Protocols in Immunology. 48:21.3:21.3.1–21.3.6.
- Published Online: 1 MAY 2002
- Published Print: APR 2002
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications (e.g., brightfield, phase-contrast, differential interference contrast, and fluorescence epi-illumination), and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The principles of microscopy and step-by-step alignment and adjustment procedures are described in this unit.