UNIT 22B.1 Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells

  1. Hideo Ema1,
  2. Yohei Morita1,
  3. Hiromitsu Nakauchi1,
  4. Yumi Matsuzaki2

Published Online: 1 JUL 2005

DOI: 10.1002/0471142735.im22b01s67

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Ema, H., Morita, Y., Nakauchi, H. and Matsuzaki, Y. 2005. Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells. Current Protocols in Immunology. 67:B:22B.1:22B.1.1–22B.1.13.

Author Information

  1. 1

    The University of Tokyo, Tokyo, Japan

  2. 2

    Keio University School of Medicine, Tokyo, Japan

Publication History

  1. Published Online: 1 JUL 2005
  2. Published Print: JUN 2005


Protocols for the purification of CD34KSL cells, CD34+KSL cells, SP cells, and Tip-SP CD34KSL cells are described. Mouse hematopoietic stem cells are highly enriched in the CD34KSL and SP fractions. These two populations overlap one another. A nearly homogenous population of HSCs can be obtained by isolating Tip-SP CD34KSL cells. One of the earliest progenitor cell populations is CD34+KSL cells.


  • hematopoietic stem cells;
  • hematopoietic progenitor cells;
  • SP cells