UNIT 22B.1 Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells
Published Online: 1 JUL 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Ema, H., Morita, Y., Nakauchi, H. and Matsuzaki, Y. 2005. Isolation of Murine Hematopoietic Stem Cells and Progenitor Cells. Current Protocols in Immunology. 67:B:22B.1:22B.1.1–22B.1.13.
- Published Online: 1 JUL 2005
- Published Print: JUN 2005
Protocols for the purification of CD34−KSL cells, CD34+KSL cells, SP cells, and Tip-SP CD34−KSL cells are described. Mouse hematopoietic stem cells are highly enriched in the CD34−KSL and SP fractions. These two populations overlap one another. A nearly homogenous population of HSCs can be obtained by isolating Tip-SP CD34−KSL cells. One of the earliest progenitor cell populations is CD34+KSL cells.
- hematopoietic stem cells;
- hematopoietic progenitor cells;
- SP cells