UNIT 22F.2 In Vitro Differentiation and Measurement of B Cell Progenitor Activity in Culture

  1. Taku Kouro,
  2. Takafumi Yokota,
  3. Robert Welner,
  4. Paul W. Kincade

Published Online: 1 MAY 2005

DOI: 10.1002/0471142735.im22f02s66

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kouro, T., Yokota, T., Welner, R. and Kincade, P. W. 2005. In Vitro Differentiation and Measurement of B Cell Progenitor Activity in Culture. Current Protocols in Immunology. 66:F:22F.2:22F.2.1–22F.2.12.

Author Information

  1. Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma

Publication History

  1. Published Online: 1 MAY 2005
  2. Published Print: APR 2005


As well as other blood cells, B lymphocytes originate from hematopoietic stem cells. However, it is not fully understood how their production is controlled. In the serum-free, stromal-cell-free B cell differentiation culture described here, early steps of the B lineage differentiation process are reproduced under defined conditions. This assay is useful for examining the direct effects of various soluble factors on B cell progenitors because it does not contain stromal cells or unknown factors. Additionally, this assay yields sufficient cloning to measure B cell progenitors from single cell cultures. Stromal cell coculture assays are also described that cover a wider category of precursors such as human B cell progenitors.


  • B lymphocyte;
  • progenitors;
  • differentiation;
  • culture;
  • stromal cell;
  • cytokine