UNIT 22F.2 In Vitro Differentiation and Measurement of B Cell Progenitor Activity in Culture
Published Online: 1 MAY 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Kouro, T., Yokota, T., Welner, R. and Kincade, P. W. 2005. In Vitro Differentiation and Measurement of B Cell Progenitor Activity in Culture. Current Protocols in Immunology. 66:F:22F.2:22F.2.1–22F.2.12.
- Published Online: 1 MAY 2005
- Published Print: APR 2005
As well as other blood cells, B lymphocytes originate from hematopoietic stem cells. However, it is not fully understood how their production is controlled. In the serum-free, stromal-cell-free B cell differentiation culture described here, early steps of the B lineage differentiation process are reproduced under defined conditions. This assay is useful for examining the direct effects of various soluble factors on B cell progenitors because it does not contain stromal cells or unknown factors. Additionally, this assay yields sufficient cloning to measure B cell progenitors from single cell cultures. Stromal cell coculture assays are also described that cover a wider category of precursors such as human B cell progenitors.
- B lymphocyte;
- stromal cell;