UNIT 22F.3 Measurement of Natural Killer Cell Progenitor Activity in Culture

  1. Taku Kouro,
  2. Takafumi Yokota,
  3. Robert Welner,
  4. Paul W. Kincade

Published Online: 1 MAY 2005

DOI: 10.1002/0471142735.im22f03s66

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kouro, T., Yokota, T., Welner, R. and Kincade, P. W. 2005. Measurement of Natural Killer Cell Progenitor Activity in Culture. Current Protocols in Immunology. 66:F:22F.3:22F.3.1–22F.3.4.

Author Information

  1. Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma

Publication History

  1. Published Online: 1 MAY 2005
  2. Published Print: APR 2005


Natural killer cells are morphologically related to and have progenitors in common with B and T lymphocytes. In this unit, protocols describe how to induce natural killer cell differentiation from prolymphocytes. Since NK cell differentiation cultures also support B cell differentiation, this protocol is extremely useful for seeking the B and NK cell branch point in their differentiation pathway.


  • Natural killer cells;
  • progenitors;
  • differentiation;
  • culture;
  • cytokine