UNIT 22F.4 Differentiation of Peripheral Blood Monocytes into Dendritic Cells

  1. David W. O'Neill,
  2. Nina Bhardwaj

Published Online: 1 JUL 2005

DOI: 10.1002/0471142735.im22f04s67

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

O'Neill, D. W. and Bhardwaj, N. 2005. Differentiation of Peripheral Blood Monocytes into Dendritic Cells. Current Protocols in Immunology. 67:F:22F.4:22F.4.1–22F.4.9.

Author Information

  1. New York University School of Medicine, New York, New York

Publication History

  1. Published Online: 1 JUL 2005
  2. Published Print: JUN 2005


Dendritic cells (DCs) are potent antigen-presenting cells (APC) that are important in the initiation and control of cellular immune responses. Commonly used in T cell–stimulation experiments, DCs are typically “matured” in vitro with microbial products or proinflammatory cytokines, and then loaded with antigens from any number of sources, including peptides, whole proteins, cell lysates, RNA, microbes, or killed tumor cells. This unit presents a simple and commonly used method for the generation of mature human dendritic cells—differentiating them from peripheral blood monocytes.


  • antigen presenting cells;
  • dendritic;
  • human;
  • monocytes