UNIT 22F.8 Differentiation and Characterization of T Cells
Published Online: 1 NOV 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Robertson, P. and Scadden, D. T. 2005. Differentiation and Characterization of T Cells. Current Protocols in Immunology. 69:F:22F.8:22F.8.1–22F.8.8.
- Published Online: 1 NOV 2005
- Published Print: OCT 2005
This paper outlines current standard methods of inducing T lymphopoiesis in vitro and in vivo. Reference is made to both murine and human systems. In addition to differentiation assays, methods to optimally characterize output cells are discussed. In bringing together a number of existing protocols, many techniques important in investigating T cell development can be reviewed in one place.
- T cell development;
- Fetal thymic organ culture (FTOC);
- Intrathymic injection;
- T cell characterization