UNIT 22F.8 Differentiation and Characterization of T Cells

  1. Paul Robertson,
  2. David T. Scadden

Published Online: 1 NOV 2005

DOI: 10.1002/0471142735.im22f08s69

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Robertson, P. and Scadden, D. T. 2005. Differentiation and Characterization of T Cells. Current Protocols in Immunology. 69:F:22F.8:22F.8.1–22F.8.8.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2005
  2. Published Print: OCT 2005


This paper outlines current standard methods of inducing T lymphopoiesis in vitro and in vivo. Reference is made to both murine and human systems. In addition to differentiation assays, methods to optimally characterize output cells are discussed. In bringing together a number of existing protocols, many techniques important in investigating T cell development can be reviewed in one place.


  • T cell development;
  • Fetal thymic organ culture (FTOC);
  • Intrathymic injection;
  • T cell characterization