APPENDIX 1W Using Bioinformatics Tools for the Sequence Analysis of Immunoglobulins and T Cell Receptors

  1. Marie-Paule Lefranc

Published Online: 1 MAR 2006

DOI: 10.1002/0471142735.ima01ws71

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Lefranc, M.-P. 2006. Using Bioinformatics Tools for the Sequence Analysis of Immunoglobulins and T Cell Receptors. Current Protocols in Immunology. 71:1W:A.1W.1–A.1W.15.

Author Information

  1. IMGT, Université Montpellier II, CNRS, Montpellier, France

Publication History

  1. Published Online: 1 MAR 2006
  2. Published Print: FEB 2006


The huge potential repertoire of 1012 immunoglobulins and 1012 T cell receptors per individual results from complex mechanisms of combinatorial diversity between the variable (V), diversity (D), and junction (J) genes, nucleotide deletions and insertions (N-diversity) at the junctions and, for the immunoglobulins, somatic hypermutations. The accurate analysis of rearranged immunoglobulin and T cell receptor sequences, and the annotation of the junctions, therefore represent a huge challenge. The IMGT Scientific chart rules, based on the IMGT-ONTOLOGY concepts, were the prerequisites for the implementation of the IMGT/V-QUEST and IMGT/JunctionAnalysis tools. IMGT/V-QUEST analyzes germline V and rearranged V-J or V-D-J nucleotide sequences. IMGT/JunctionAnalysis is the first tool that automatically analyzes the complex junctions in detail. These interactive tools are easy to use and freely available on the Web (, either separately or integrated.


  • IMGT;
  • immunoglobulin;
  • T cell receptor;
  • rearrangement;
  • immunogenetics;
  • immunoinformatics;
  • junction analysis;
  • sequence diversity;
  • Collier de Perles;
  • variable gene;
  • variable domain;
  • IMGT/JunctionAnalysis