APPENDIX 2A Common Media, Buffers, and Stock Solutions

This section describes the preparation of media, buffers, and reagents used in this manual.

Culture Media

• Complete Click's medium
  
• Complete DMEM medium
  
• Complete IMDM medium
  
• Complete RPMI medium
  

Buffers and Stock Solutions

• Acids, concentrated stock solutions
  
• Alsever's solution
  
• Ammonium acetate, 10 M
  
• Carbonate buffer
  
• Denhardt solution, 100×
  
• DTT (dithiothreitol), 1 M
  
• EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0)
  
• HBSS (Hanks' balanced salt solution)
  
• HCl, 1 M
  
• 2-ME (2-mercaptoethanol), 50 mM
  
• MgCl₂, 1 M
  
• NaCl, 5 M
  
• NaOH, 10 M
  
• PBS (phosphate-buffered saline)
  
• Potassium phosphate buffer, 0.1 M
  
• SDS, 20% (w/v)
  
• Sodium acetate, 3 M
  
• Sodium acetate buffer, pH 4.0 to 5.0
  
• Sodium phosphate buffer, 0.1 M
  
• TBS (Tris-buffered saline)
  
• TE (Tris/EDTA) buffer
  
• Tris⋅Cl, 1 M