Appendix

APPENDIX 3A Monitoring Cell Growth

  1. Warren Strober

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03as21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Strober, W. 2001. Monitoring Cell Growth. Current Protocols in Immunology. 21:3A:A.3A.1–A.3A.2.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

This appendix provides two protocols for monitoring cell growth. Counting cells using a hemacytometer is tedious but it allows one to effectively distinguish live cells from dead cells (using Trypan Blue exclusion). In addition, this procedure is less subject to errors due to cell clumping or heterogeneity of cell size. The use of an electronic cell counter is quicker and easier than counting cells using a hemacytometer. However, an electronic cell counter as currently constructed does not distinguish live from dead cells in a reliable fashion and is subject to error due to the presence of cell clumps. Overall, the electronic cell counter is best reserved for repetitive and rapid counting of fresh peripheral blood cells and should be used with caution when counting cell populations derived from tissues.