Appendix

APPENDIX 3B Trypan Blue Exclusion Test of Cell Viability

  1. Warren Strober

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03bs21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Strober, W. 2001. Trypan Blue Exclusion Test of Cell Viability. Current Protocols in Immunology. 21:3B:A.3B.1–A.3B.2.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

The dye exclusion test is used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. In this test, a cell suspension is simply mixed with dye and then visually examined to determine whether cells take up or exclude dye. In the protocol presented here, a viable cell will have a clear cytoplasm whereas a nonviable cell will have a blue cytoplasm.