APPENDIX 3B Trypan Blue Exclusion Test of Cell Viability
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Strober, W. 2001. Trypan Blue Exclusion Test of Cell Viability. Current Protocols in Immunology. 21:3B:A.3B.1–A.3B.2.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
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The dye exclusion test is used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. In this test, a cell suspension is simply mixed with dye and then visually examined to determine whether cells take up or exclude dye. In the protocol presented here, a viable cell will have a clear cytoplasm whereas a nonviable cell will have a blue cytoplasm.