Appendix

APPENDIX 3D Labeling Cells in Microtiter Plates for Determination of [3H]Thymidine Uptake

  1. Ethan M. Shevach

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03ds21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Shevach, E. M. 2001. Labeling Cells in Microtiter Plates for Determination of [3H]Thymidine Uptake. Current Protocols in Immunology. 21:3D:A.3D.1.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

A number of protocols in Current Protocols in Immunology use as their end-point the determination of cell proliferation by determining the incorporation of [3H]thymidine into cellular DNA. This appendix presents a protocol in which the radioactive label is added during the last 4 to 24 hr of the culture. A semiautomated cell harvesting apparatus is then used to lyse the cells with water and precipitate the labeled DNA on glass fiber filters. The filter pads are then dried and counted by standard liquid scintillation counting techniques in a scintillation counter.