APPENDIX 3E Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures

  1. Frank W. Fitch1,
  2. Thomas F. Gajewski1,
  3. Wayne M. Yokoyama (antibiotic treatment)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03es21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Fitch, F. W., Gajewski, T. F. and Yokoyama, W. M. 2001. Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures. Current Protocols in Immunology. 21:3E:A.3E.1–A.3E.4.

Author Information

  1. 1

    University of Chicago, Chicago, Illinois

  2. 2

    University of California School of Medicine, San Francisco, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997


Mycoplasma contamination is a serious and frequent problem in the culture laboratory. Although mycoplasma contamination may be suspected by the failure of cells to thrive, the formal diagnosis rests on the detection of adenosine phosphorylase secretion by infected cell lines. This appendix describes how to test for mycoplasma contamination, and also presents methods for antibiotic treatment of infected cultures.