APPENDIX 3E Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Fitch, F. W., Gajewski, T. F. and Yokoyama, W. M. 2001. Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures. Current Protocols in Immunology. 21:3E:A.3E.1–A.3E.4.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
Mycoplasma contamination is a serious and frequent problem in the culture laboratory. Although mycoplasma contamination may be suspected by the failure of cells to thrive, the formal diagnosis rests on the detection of adenosine phosphorylase secretion by infected cell lines. This appendix describes how to test for mycoplasma contamination, and also presents methods for antibiotic treatment of infected cultures.