Appendix

APPENDIX 3F Obtaining Human Peripheral Blood Cells

  1. Warren Strober

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03fs21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Strober, W. 2001. Obtaining Human Peripheral Blood Cells. Current Protocols in Immunology. 21:3F:A.3F.1–A.3F.2.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

Peripheral blood is the chief source of mononuclear cells (lymphocytes and monocytes) for immunologic studies of humans. This appendix presents protocols for obtaining blood by simple venipuncture when relatively small amounts of blood (10 to 100 ml) are necessary or by lymphapheresis when large amounts (300 to 5000 ml) are necessary. Cells collected by these procedures can be further separated by techniques described in Chapter 7.