APPENDIX 3H Dialysis and Concentration of Protein Solutions

  1. Sarah M. Andrew1,
  2. Julie A. Titus2,
  3. Louis Zumstein3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03hs21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Andrew, S. M., Titus, J. A. and Zumstein, L. 2001. Dialysis and Concentration of Protein Solutions. Current Protocols in Immunology. 21:3H:A.3H.1–A.3H.5.

Author Information

  1. 1

    Lancaster University, Lancaster, United Kingdom

  2. 2

    National Cancer Institute, Bethesda, Maryland

  3. 3

    Introgen Therapeutics, Inc., Houston, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997


Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. This appendix describes dialysis of large- and small-volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight. A Support Protocol describes preparation of membranes for dialysis and discusses issues related to the selection of membranes including commercial kits.