APPENDIX 3H Dialysis and Concentration of Protein Solutions
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Andrew, S. M., Titus, J. A. and Zumstein, L. 2001. Dialysis and Concentration of Protein Solutions. Current Protocols in Immunology. 21:3H:A.3H.1–A.3H.5.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. This appendix describes dialysis of large- and small-volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight. A Support Protocol describes preparation of membranes for dialysis and discusses issues related to the selection of membranes including commercial kits.