APPENDIX 3I Column Chromatography of Antibodies and Fragments

  1. John A. Smith

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03is21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Smith, J. A. 2001. Column Chromatography of Antibodies and Fragments. Current Protocols in Immunology. 21:3I:A.3I.1–A.3I.11.

Author Information

  1. University of Alabama at Birmingham, Birmingham, Alabama

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997


Since both size-exclusion (SE) chromatography (also known as gel filtration) and ion-exchange (IEX) chromatography are the fundamental methods on which antibody and fragment purifications are based, they are dealt with in detail in this appendix. The procedures for preparing the column matrix, for pouring the chromatography column, and for estimating the appropriate size of the column for the amount of antibody and fragment that are to be purified are all described.