APPENDIX 3I Column Chromatography of Antibodies and Fragments
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Smith, J. A. 2001. Column Chromatography of Antibodies and Fragments. Current Protocols in Immunology. 21:3I:A.3I.1–A.3I.11.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
Since both size-exclusion (SE) chromatography (also known as gel filtration) and ion-exchange (IEX) chromatography are the fundamental methods on which antibody and fragment purifications are based, they are dealt with in detail in this appendix. The procedures for preparing the column matrix, for pouring the chromatography column, and for estimating the appropriate size of the column for the amount of antibody and fragment that are to be purified are all described.