APPENDIX 3K Silanizing Glassware

  1. Brian Seed

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03ks21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Seed, B. 2001. Silanizing Glassware. Current Protocols in Immunology. 21:3K:A.3K.1–A.3K.2.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997


Glassware is silanized (siliconized) to prevent adsorption of solute to glass surfaces or to increase its hydrophobicity. This is particularly important with low concentrations of particularly “sticky“ solutes such as single-stranded nucleic acids or proteins. This appendix provides a protocol for treating glassware.