APPENDIX 3L Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Gallagher, S. R. 2001. Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols in Immunology. 21:A.3L.1–A.3L.7.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers using molecular biology techniques. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques are presented in this appendix. These three procedures cover a range from 5 to 10 ng DNA/ml to 50mg DNA/ml.