Appendix

APPENDIX 3L Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy

  1. Sean R. Gallagher

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03ls21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Gallagher, S. R. 2001. Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy. Current Protocols in Immunology. 21:3L:A.3L.1–A.3L.7.

Author Information

  1. Hoefer Scientific Instruments, San Francisco, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers using molecular biology techniques. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques are presented in this appendix. These three procedures cover a range from 5 to 10 ng DNA/ml to 50mg DNA/ml.