Appendix

APPENDIX 3N Transformation of E. coli by Electroporation

  1. Sherie L. Morrison

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.ima03ns21

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Morrison, S. L. 2001. Transformation of E. coli by Electroporation. Current Protocols in Immunology. 21:3N:A.3N.1–A.3N.4.

Author Information

  1. University of California Los Angeles, Los Angeles, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1997

Abstract

Using electroporation to transform Escherichia coli results in transformation efficiencies greater than can be obtained using the best chemical methods. It is easy to obtain transformation efficiencies 108 per milligram DNA and efficiencies of 1010 have been reported. This appendix describes a procedure for electroporation that can be used to transform many different types of bacteria.