APPENDIX 3N Transformation of E. coli by Electroporation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Morrison, S. L. 2001. Transformation of E. coli by Electroporation. Current Protocols in Immunology. 21:3N:A.3N.1–A.3N.4.
- Published Online: 1 MAY 2001
- Published Print: MAR 1997
Using electroporation to transform Escherichia coli results in transformation efficiencies greater than can be obtained using the best chemical methods. It is easy to obtain transformation efficiencies 108 per milligram DNA and efficiencies of 1010 have been reported. This appendix describes a procedure for electroporation that can be used to transform many different types of bacteria.