UNIT 1.3 Strategies for Genotyping
Published Online: 1 JAN 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Crawford, D. C. and Dilks, H. H. 2011. Strategies for Genotyping. Current Protocols in Human Genetics. 68:1.3:1.3.1–1.3.22.
- Published Online: 1 JAN 2011
- Published Print: JAN 2011
The identification of genomic loci linked to or associated with human disease has been greatly facilitated by the evolution of genotyping strategies and techniques. The success of these strategies continues to be based upon clear clinical assessment, accurate sample handling, and careful data management, but also increasingly upon experimental design. Technological advances in the field of genotyping have permitted increasingly complex and large population studies to be performed. An understanding of publicly available genetic variation databases, including an awareness of the limitations of these data, and an appreciation of the strategic approaches that should be used to exploit this information will provide tremendous insight for researchers are aiming to utilize this accessible technology. As genome-wide association studies (GWAS) and Next Generation (NextGen) sequencing become the mainstays of genetic analyses, it is important that their technical strengths and limitations, as well as their impact on study design, be understood before use in a linkage or genetic association study. Curr. Protoc. Hum. Genet. 68:1.3.1-1.3.22 © 2011 by John Wiley & Sons, Inc.