Unit

UNIT 1.6 Single-Sperm Typing

  1. Sigbjorn Lien1,
  2. Joanna Szyda2,
  3. Esther P. Leeflang3,
  4. Rene Hubert3,
  5. Lin Zhang3,
  6. Karin Schmitt3,
  7. Norman Arnheim3

Published Online: 1 MAY 2002

DOI: 10.1002/0471142905.hg0106s32

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Lien, S., Szyda, J., Leeflang, E. P., Hubert, R., Zhang, L., Schmitt, K. and Arnheim, N. 2002. Single-Sperm Typing. Current Protocols in Human Genetics. 32:1.6:1.6.1–1.6.18.

Author Information

  1. 1

    Agricultural University of Norway, Aas, Norway

  2. 2

    Wroclaw Agricultural University, Wroclaw, Poland

  3. 3

    University of Southern California, Los Angeles, California

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: JAN 2002

Abstract

This unit presents protocols for sperm isolation using two different methods, amplification of simple sequence-length polymorphisms (SSLP) and/or single nucleotide polymorphisms (SNP) from single cells or whole genome-amplified single cells using primer extension preamplification (PEP), and discusses the statistical analysis of sperm-typing recombination data. Newer methods for studying recombination over very short distances (a few kilobases) using total sperm DNA and allele-specific PCR are also discussed.