UNIT 1.6 Single-Sperm Typing
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Lien, S., Szyda, J., Leeflang, E. P., Hubert, R., Zhang, L., Schmitt, K. and Arnheim, N. 2002. Single-Sperm Typing. Current Protocols in Human Genetics. 32:1.6:1.6.1–1.6.18.
- Published Online: 1 MAY 2002
- Published Print: JAN 2002
This unit presents protocols for sperm isolation using two different methods, amplification of simple sequence-length polymorphisms (SSLP) and/or single nucleotide polymorphisms (SNP) from single cells or whole genome-amplified single cells using primer extension preamplification (PEP), and discusses the statistical analysis of sperm-typing recombination data. Newer methods for studying recombination over very short distances (a few kilobases) using total sperm DNA and allele-specific PCR are also discussed.